| 生物活性： |
靶点：Bacterial；Fungal；Influenza Virus；Antibiotic；ER Stress
通路：Anti-infection；ER Stress
背景说明：Tunicamycin是一种同源核苷抗生素的混合物, 可抑制 N-糖基化并阻断 GlcNAc 磷酸转移酶 (GPT)。Tunicamycin 引起细胞内质网 (ER) 中未折叠蛋白的积累并诱导 ER 应激，并导致 DNA 合成受阻和 G1 期细胞周期停滞。
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| 其它标识： |
EC：EINECS 601-012-4
MDL：MFCD00065709
SMILES：O=C1NC(N([C@H]2[C@H](O)[C@H](O)[C@@H](C(C[C@@H]3[C@H](O)[C@H](O)[C@@H](NC(/C=C/[(CH2) 8-11]C(C)C)=O)[C@H](O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4NC(C)=O)O3)O)O2)C=C1)=O
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| 基本信息： |
CAS：11089-65-9
中文名称：衣霉素
英文名称：Tunicamycin
分子式：Mixture
规格：1mg
溶解性：Soluble in DMSO
纯度：≥98%
外观(性状)：White to light yellow Solid
储存条件：Powder:-20℃，2 years;Insolvent(母液):-20℃，6 months;-80℃，1 year
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| 靶点： |
Bacterial；Fungal；Influenza Virus；Antibiotic；ER Stress |
产品详情
Tunicamycin是一种抗生素, 可抑制 N-糖基化并阻断 GlcNAc 磷酸转移酶 (GPT)。Tunicamycin 引起细胞内质网 (ER) 中未折叠蛋白的积累并诱导 ER 应激，并导致 DNA 合成受阻和 G1 期细胞周期停滞。
衣霉素是一种同源核苷抗生素的混合物, 为四种异构体A, B, C, D的混合物：
异构体A, n=8, C37H60N4O16, 816.90
异构体B, n=9, C38H62N4O16, 830.93
异构体C, n=10, C39H64N4O16, 844.95
异构体D, n=11, C40H66N4O16, 858.99
使用本产品的案例（仅供参考）
In Vitro
Cell（Buffalo Ovarian Granulosa Cells; 1~5 µg/mL tunicamycin; 37◦C, 5% CO2, 4–48 h）
Briefly, the culture medium was removed and replaced with the culture medium consisting of diluted tunicamycin (TM) (Solarbio, Beijing, China). GCs were then subjected to increasing doses of TM at concentrations of 1, 1.5, 2, 2.5, and 5 µg/mL and cultured at 37◦C for approximately 24–48 h in a 5% CO2 incubator.
来源文献：Yang W, Liu R, Sun Q, Huang X, Zhang J, Huang L, Zhang P, Zhang M, Fu Q. Quercetin Alleviates Endoplasmic Reticulum Stress-Induced Apoptosis in Buffalo Ovarian Granulosa Cells. Animals (Basel). 2022 Mar 20;12(6):787. doi: 10.3390/ani12060787. PMID: 35327186; PMCID: PMC8944572.
Cell（ human NSCLC cell lines A549, PC9, and H1299；0.5-2 µM Tunicamycin/TM；24-72h）
0.5, 1, and 2 µM TM were used for 48 h or 2 µM TM was used for 24, 48, and 72 h to induce ER stress. Ten micromolar MG132 was used for 6 h prior to harvesting the cells, and 50 μg·mL−1 CHX was used for indicated time to detect protein degradation. Ten micromolar Radezolid was used to inhibit RRBP1 expression.
来源文献：Wang W, Wang M, Xiao Y, Wang Y, Ma L, Guo L, Wu X, Lin X, Zhang P. USP35 mitigates endoplasmic reticulum stress-induced apoptosis by stabilizing RRBP1 in non-small cell lung cancer. Mol Oncol. 2022 Apr;16(7):1572-1590. doi: 10.1002/1878-0261.13112. Epub 2021 Oct 18. PMID: 34618999; PMCID: PMC8978513.
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