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埃文斯蓝

品牌：北京金优科技有限公司

货号	规格	包装/纯度	价格	货期	操作
JY-2025IT99100mg	100mg	≥85%	¥640.00 会员价请登录

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生物活性：	靶点：GluR 通路：Neuronal Signaling 背景说明：Evans Blue是一种非渗透染料，可用于检查细胞的活力和研究血脑屏障的通透性。Evans Blue也是一种有效的L-谷氨酸摄取抑制剂。
其它标识：	EC：EINECS 206-242-5 MDL：MFCD00004021 SMILES：[O-]S(C1=CC(S([O-])(=O)=O)=C2C=CC(/N=N/C3=CC=C(C4=CC=C(/N=N/C5=C(O)C6=C(N)C(S([O-])(=O)=O)=CC(S([O-])(=O)=O)=C6C=C5)C(C)=C4)C=C3C)=C(O)C2=C1N)(=O)=O.[Na+].[Na+].[Na+].[Na+] InChIKey：ATNOAWAQFYGAOY-UHFFFAOYSA-J InChI：InChI=1S/C34H28N6O14S4.4Na/c1-15-11-17(3-7-21(15)37-39-23-9-5-19-25(55(43,44)45)13-27(57(49,50)51)31(35)29(19)33(23)41)18-4-8-22(16(2)12-18)38-40-24-10-6-20-26(56(46,47)48)14-28(58(52,53)54)32(36)30(20)34(24)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4 PubChem CID：9409
基本信息：	CAS：314-13-6 中文名称：埃文斯蓝英文名称：Evans Blue 别名：伊文思蓝;C.I.23860;Direct Blue 53 分子式：C₃₄H₂₄N₆Na₄O₁₄S₄ 分子量：960.81 规格：100mg ; 10mM*1mL in DMSO ; 500mg 溶解性：Soluble in Water/DMSO ≥5mg/mL 纯度：≥85% 外观(性状)：Reddish brown Solid 储存条件：Powder:2-8℃，2 years;Insolvent(母液):-20℃，6 months;-80℃，1 year
靶点：	GluR

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Evans Blue又称偶氮蓝，与台盼蓝都是细胞活性染料，常用于检测细胞膜的完整性和细胞是否存活。活细胞因有外排功能而无法被<a target="_blank" title=" href="https://www.solarbio.com/goods-47209.html" style="margin: 0px; padding: 0px; box-sizing: border-box; text-decoration-line: none; cursor: pointer; color: black; outline: none; font-family: MicrosoftYaHeiUI; background-color: rgb(255, 255, 255);">埃文斯蓝染成蓝色，而死细胞会被染成淡蓝色。因此可以通过此方法在显微镜下区分死细胞与活细胞，但无法区分死亡与坏死。

Evans Blue是一种非渗透染料，在质膜存在损伤时，染料可进入细胞质和核中并将它们染成蓝色。可用于检查细胞的活力和研究血脑屏障的通透性。

Evans Blue也是一种有效的L-谷氨酸摄取抑制剂，通过膜结合的兴奋性氨基酸转运蛋白 (EAAT)。

使用本产品的案例（仅供参考）

In Vivo

Evans blue 染色（尾静脉注射；2% Evans blue；1mL）

Post BMS or PLS implantation, ≥ three healthy SD rats were randomly selected and respectively anesthetized for periods of 1 week and 1, 3 months. The first involved a 10% chloral hydrate by intraperitoneal injection, and then a 1 mL 2% Evans blue (Solarbio, CAS: 314-13-6) was injected by tail intravenous injection. These rats were subsequently sacrificed 1 h after the dyeing cycle at which point the heart was perfused with 0.9% saline containing one heparin sodium salt (Solarbio, CAS: 9041-08-1). At this time, blood vessels with implanted scaffolds were removed. After removing excess tissue fat, blood vessels were dissected longitudinally, fixed with 4% paraformaldehyde. Subsequently, these blood vessels were stored at 4 ◦C to be used for collecting images of the endocardium coloration.

来源文献：Yin T, et al. Two-stage degradation and novel functional endothelium characteristics of a 3-D printed bioresorbable scaffold. Bioact Mater. 2021 Aug 24;10:378-396. doi: 10.1016/j.bioactmat.2021.08.020.

In Vitro

Cell viability（生菜幼苗的根段, 0.25% (w/v) Evans blue, 1h, 600nm）

Root cell viability was also quantitatively evaluated by detecting the relative uptake of Evans blue. Root segments of the treated lettuce seedlings were soaked in 0.25% (w/v) Evans blue (Solarbio, Beijing, China) for 1 h at 25°C. The stained roots were washed with distilled H2O, and then soaked in 1 mL N,N-dimethylformamide for 24 h at 25°C in darkness to extract the Evans blue that had been absorbed into the lettuce roots. Absorbance of the released Evans blue was measured at 600 nm by a spectrophotometer. The relative Evans blue uptake was calculated as the ratio between the OD values of the treated group and the control.

来源文献：Yan ZQ, Tan J, Guo K, Yao LG. Phytotoxic mechanism of allelochemical liquiritin on root growth of lettuce seedlings. Plant Signal Behav. 2020 Oct 2;15(10):1795581. doi: 10.1080/15592324.2020.1795581. Epub 2020 Jul 21.

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