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背景说明：Peptide C105Y TFA，基于 β-抗胰蛋白酶 359-374 残基氨基酸序列合成的细胞穿透性肽，能增强 DNA 纳米粒的基因表达。 Peptide C105Y TFA, a synthetic and cell-penetrating peptide based on the amino acid sequence corresponding to residues 359-374 of β-antitrypsin, enhances gene expression from DNA nanoparticles.
In Vitro：When human hepatoma cells (HuH7) were incubated with fluorescently labeled C105Y for as little as 3 min, C105Y displays nuclear and cytoplasmic staining with enrichment of fluorescent signal in the nucleus and nucleolus. C105Y peptide is routed to the nucleolus very rapidly in an energy-dependent fashion, whereas membrane translocation and nuclear localization are energy-independent. When tested the involvement of known endocytosis pathways in uptake and trafficking of this peptide, C105Y peptide is internalized by a clathrin- and caveolin-independent pathway, although lipid raft-mediated endocytosis may play a role in peptide intracellular trafficking.
参考文献：[1] M. Rhee and P. Davis J. Biol. Chem. 281, 1233 (2006).
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