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潮霉素B
品牌：北京金优科技有限公司
货号 规格 包装/纯度 价格 货期 操作
JY-2025IC86100mg 100mg ≥90%
¥380.00
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生物活性： 靶点：Bacterial;Fungal
通路：Anti-infection
背景说明：是抑制原核和真核细胞的氨基糖苷类抗生素,通过干扰移位、促进70S核糖体的误译来抑制蛋白质合成。
生物活性：Hygromycin B is an aminoglycoside antibiotic active against prokaryotic and eukaryotic cells.[1-3]
In Vitro：Hygromycin B，an aminocyclitol antibiotic that strongly inhibits both 70S and 80S ribosomes，is synthesized by Streptomyces hygroscopicus[1]. Hygromycin B at 0.38 mM concentration completely halts yeast cell growth in rich media，presumably by preventing protein synthesis by cytoplasmic ribosomes. Polypeptide synthesis in cell-free extracts from rabbit reticulocytes，wheat germ and yeast is strongly blocked by low concentrations ofhygromycin B. The antibiotic inhibits peptide chain elongation by yeast polysomes by preventing elongation factor EF-2-dependent translocation. The inhibition of translocation by hygromycin B might result from the stabilization of peptidyl-tRNA bound to the ribosomal acceptor site[2].
In Vivo：Hygromycin B inhibits protein synthesis by blocking ribosomal translocation without causing significant misreading in vivo[3]. Constitutive expression of the bacterial hygR gene in transgenic mice in vivo confers resistance to hygromycin B[4].
动物实验：Hygromycin B is dissolved in sterile water. The mice C57BL/6J-TgN(pPWL512hyg)1Ems carrying hygR are treated with a single dose of hygromycin B i.p. at doses that starts at 2.7 mg/kg and increases by 50% for each consecutive dose. Control wild-type C57BL/6J mice are treated with the same volume of sterile saline. Total volume injected is 0.5 mL. The health status and body weights of animals are monitored daily for 10 consecutive days[4].
数据来源文献：[1]. Pardo JM， et al. Biochemical basis of resistance to hygromycin B in Streptomyces hygroscopicus--the producing organism. J Gen Microbiol. 1985 Jun;131(6):1289-98.
[2]. González A， et al. Studies on the mode of action of hygromycin B， an inhibitor of translocation in eukaryotes. Biochim Biophys Acta. 1978 Dec 21;521(2):459-69.
[3]. Pfister P， et al. Role of 16S rRNA Helix 44 in Ribosomal Resistance to Hygromycin B. Antimicrob Agents Chemother. 2003 May;47(5):1496-502.
其它标识： EC：EINECS 250-545-5
MDL：MFCD00036846
SMILES：O[C@H]([C@H]1O)C2(O[C@@]([C@@H](N)CO)([H])[C@@H]1O)O[C@@]([C@@H](O[C@@H]3CO)O[C@@]([C@H]([C@H](N)C4)O)([H])[C@@H]([C@H]4NC)O)([H])[C@@]([C@H]3O)([H])O2
InChIKey：GRRNUXAQVGOGFE-XKIAHZFYSA-N
InChI：InChI=1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1
PubChem CID：23777801
基本信息： CAS：31282-04-9
中文名称：潮霉素B
英文名称：Hygromycin B
别名：Hygrovetine
分子式：C20H37N3O13
分子量：527.52
规格：10mM*1mL in Water ; 100mg ; 1g ; 200mg
溶解性：Soluble in Water ≥100mg/mL
纯度：≥90%
外观(性状)：Off-white to yellow Solid
储存条件：Powder:2-8℃，2 years;Insolvent(母液):-20℃，6 months;-80℃，1 year
靶点： Bacterial;Fungal

产品详情

使用本产品的案例（仅供参考）

In Vitro

Cell (HEK293T cells; 100-400 mg/mL Hygromycin B)

In brief, the plasmid DNA was isolated from the TOP10 F' Escherichia coli containing pcDNA3.1 /Hygro (+)-ASPH using  plasmid miniprep kit according to the manufacturer's protocol.  The plasmid was then linearized using FspI digestion. HEK293T cells (2 × 105 cells/well) were seeded in 6-well cell culture plates to give optimal confluency (70%) at the time of transfection. Transfection was carried out using 4 μg of plasmid DNA and 8 μL of transfection reagent. After three days, the medium was replaced with a fresh medium containing 100 mg/mL hygromycin B (Solarbio Science & Technology, China). The plate was fed with fresh selective medium every 4 days and was screened for resistant cells remained in different parts of the plate. The detected resistant cells were monitored for 4 weeks to spot separated subclones with the ASPH expression potential.  Five selected subclones were carefully scraped off the plate using sterile scrapers and transferred to separate flasks. After 2 days, the cells were trypsinized and redistributed in the flask. The mRNA expression level of each flask was evaluated as described below (subsection qRT-PCR) to select the subclones with the highest ASPH expression. Then, the concentration of hygromycin B was gradually increased in the selected flasks to reach 400 mg/mL after 4 weeks of culture.

来源文献：Bakhtiari H, Palizban AA, Khanahmad H, Mofid MR. An innovative cell selection approach in developing human cells overexpressing aspartyl/asparaginyl β-hydroxylase. Res Pharm Sci. 2020 Jul 3;15(3):291-299.doi: 10.4103/1735-5362.288436.


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