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背景说明：Tyr-W-MIF-I, a member of the Tyr-MIF-I family, was isolated from the frontal cortex of human brain tissue as well as from bovine hypothalami. It can act as an opiate agonist as well as an antagonist. As an opiate agonist, it was more potent than the other structurally similar peptides, including Tyr-MIF-I.
In Vivo：Tyr-W-MIF-1 (i.c.v，5 μL, 200 μg) 诱导大鼠镇痛时间延长。
Tyr-W-MIF-1 (i.t., 0.75 μg) 诱导镇痛，并被 Naloxone 逆转。
In Vitro：Tyr-W-MIF-1 (1-10 μM) 抑制 LC 神经元的自发放电。
Tyr-W-MIF-1 减弱 Morphine (3 μM) 诱导的 SH-SY5Y 人神经母细胞瘤细胞中 mu 和 delta 受体的下调。
Tyr-W-MIF-1 (0.3 μM) 抑制3H-DAMGO (对μ阿片受体有选择性) 与大鼠脑的结合。
参考文献：[1] L.Hackler et al., Neuropeptides, 24, 159(1993).
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