| Elisa信息： |
Swiss Prot：Q91Z84
Gene ID：155140
检测原理：Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗大鼠IL-23单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的大鼠IL-23会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗大鼠IL-23抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的大鼠IL-23发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的大鼠IL-23，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的大鼠IL-23浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中大鼠IL-23的浓度。
背景说明：Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. The rat p19 cDNA encodes a 196 amino acid (aa) residue precursor protein with a putative 19 aa signal peptide and 177 aa mature protein. The mature rat p19 protein shares 88%, 78%, 76%, 75%, 71%, and 70% aa sequence identity with mouse, human, canine, equine, guinea pig, and bovine, respectively. Activated macrophages and dendritic cells express p19 and p40 concurrently to produce IL-23. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12R beta 1) and the IL-23-specific receptor subunit (IL-23R). IL-23 and IL-12 have overlapping but distinct biological activities. IL-12 drives development of Th1 cells and induces production of IFN-gamma by NK cells; IL-23 induces proliferation of Th17 cells and CD4+ memory T cells distinct from Th1 which produce IL-17, a potent proinflammatory cytokine. IL-23 also drives IL-17 production by NK cells and neutrophils. While both IL-12 and IL-23 pathways respond to infectious agents, the IL-23 - IL-17 immune pathway induces the earliest recruitment of neutrophils to the site of infection while the more classic host defense and cytotoxic response is stimulated by IL-12. Dysregulation of the IL-23 - IL-17 immune pathway has a key role in organ-specific autoimmune inflammatory tissue destruction.
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