| Elisa信息： |
Gene ID：24446
检测原理：Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗大鼠HGF单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的大鼠HGF会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗大鼠HGF抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的大鼠HGF发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的大鼠HGF，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的大鼠HGF浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中大鼠HGF的浓度。
背景说明：HGF (Hepatocyte Growth Factor, Scatter Factor) induces the proliferation and migration of epithelial cells, hepatocytes, chondrocytes, keratinocytes, melanocytes, endothelial cells, and many tumor cells. During organogenesis and tissue repair, HGF promotes epithelial/endothelial morphogenesis by inducing cell scattering and branching tubulogenesis. It also supports insulin production by pancreatic beta cells, neuronal survival, and immune tolerance. HGF is secreted as a propeptide that is activated by uPA or HGF Activator at sites of tissue damage. Its signaling through the receptor HGF R/c-MET is enhanced by its prior binding to heparan sulfate proteoglycans. The serum levels of HGF are elevated in a wide range of pathologies including liver damage, acute kidney failure, myocardial infarction, type 1 diabetes, obesity, and cancer, as well as in the synovial fluid of rheumatoid arthritis patients.
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| 基本信息： |
中文名称：大鼠肝细胞生长因子检测试剂盒
英文名称：Rat HGF ELISA Kit
别名：deafness,autosomal recessive39; DFNB39; fibroblast-derived tumor cytotoxic factor; F-TCF; HGFB; HPTA; lung fibroblast-derived mitogen; Scatter factor; SFhepatopoeitin-A
储存条件：2-8℃，6 months
规格：96T ; 48T
检测目标：HGF
样品类型：血清/血浆/细胞培养上清
反应性：Rat
应用：ELISA
反应类型：Sandwich-ELISA
定量/定性：定量检测
检测范围：78.12-5000 pg/ml
灵敏度：39 pg/ml
特异性：不与HGFActivat，人的HGF,HGFActivator,HGFR/FcChimera,MSP,MSPβchain等反应。
精密度：板内，板间变异系数均<10%
回收率：80%-120%
反应时间：1.3h
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