| Elisa信息： |
Swiss Prot：O55145
Gene ID：89808
检测原理：Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗大鼠CX3CL1单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的大鼠CX3CL1会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗大鼠CX3CL1抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的大鼠CX3CL1发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的大鼠CX3CL1，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的大鼠CX3CL1浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中大鼠CX3CL1的浓度。
背景说明：CX3CL1/Fractalkine is a transmembrane adhesion protein with a chemokine domain separated from the membrane by a mucin stalk. It is upregulated on many cell types during inflammation. The chemokine and mucin regions can be shed as a soluble chemokine that signals through the receptor CX3CR1. During extravasation, membrane-bound CX3CL1 traps leukocytes, then is cleaved to allow diapedesis. Soluble CX3CL1 protects neurons from microglial neurotoxicity, recruits macrophages for wound healing, recruits osteoclast precursors for bone resorption, and contributes to pathogenesis in coronary artery disease.
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