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PNPG

品牌：北京金优科技有限公司

货号	规格	包装/纯度	价格	货期	操作
JY-2025LO57500mg	500mg	HPLC≥98%	¥480.00 会员价请登录

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产品详情

生物活性：	靶点：Others 通路：Others 背景说明：PNPG是显色的β-D-葡糖苷酶底物,裂解后会产生黄色溶液。
其它标识：	EC：EINECS 219-661-3 MDL：MFCD00006593 SMILES：O[C@H]1[C@@H](O[C@H](CO)[C@@H](O)[C@@H]1O)OC2=CC=C([N+]([O-])=O)C=C2 InChIKey：IFBHRQDFSNCLOZ-RMPHRYRLSA-N InChI：InChI=1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-3-1-6(2-4-7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9-,10+,11-,12-/m1/s1 PubChem CID：92930
基本信息：	CAS：2492-87-7 中文名称：对硝基苯基-β-D-吡喃葡萄糖苷英文名称：PNPG 别名：4-Nitrophenylβ-D-glucopyranoside;4-硝基苯基-β-D-吡喃葡萄糖苷分子式：C₁₂H₁₅NO₈ 分子量：301.25 规格：100mg ; 10mM*1mL in DMSO ; 1g ; 500mg 溶解性：Soluble in Water/PBS ≥30mg/mL；Soluble in DMSO ≥3mg/mL 纯度：HPLC≥98% 外观(性状)：White to yellow Solid 储存条件：Powder:-20℃，2 years;Insolvent(母液):-20℃，6 months;-80℃，1 year
靶点：	Others

产品详情

PNPG是显色的β-D-葡糖苷酶底物，裂解后会产生黄色溶液。

使用本产品的应用案例（仅供参考）

In Vitro

α-Glucosidase inhibitory assay（5 mM PNPG，37℃， 30 min, 405nm）

10 μL of α-glucosidase, 10 μL of 0.1 mg/mL Up, Up-3, Up-4, and Up-5, 80 μL of PBS buffer (0.02 mol/L, pH 6.8) and 10 μL of 5 mM PNPG were mixed in a 96-well plate, and then the mixture was incubated at 37℃ for 30 min.The presence of the product, p-nitrophenol (PNP) was monitored by measuring absorbance at 405 nm in a plate reader. Acarbose was used as a positive control. Reactions without inhibitor were also performed in parallel serving as controls. The PNP concentration was determined from a standard curve. The relative activity was expressed as a percentage of the PNP formation in the presence of acarbose or polysaccharides compared with that of the control reactions. Subsequently, the α-glucosidase inhibitory activity of acarbose, Up-3 and Up-4 at different concentrations (0.01, 0.03, 0.05, 0.08, 0.1, 0.3, 0.5, 1.0, and 2.0 mg/mL) were determined according to the method described above to calculate the IC50 value.

来源文献：Zhong QW, Zhou TS, Qiu WH, Wang YK, Xu QL, Ke SZ, Wang SJ, Jin WH, Chen JW, Zhang HW, Wei B, Wang H. Characterization and hypoglycemic effects of sulfated polysaccharides derived from brown seaweed Undaria pinnatifida. Food Chem. 2021 Mar 30;341(Pt 1):128148. doi: 10.1016/j.foodchem.2020.128148. Epub 2020 Sep 22. PMID: 33038776.

Determination of b-Glucosidase activity（2.0 mg/mL PNPG, 60s, 405nm）

Firstly, 30.0 mL of standard b-Glucosidase solutions (1.5-21.0 U/mL) were diluted by 50.0 mL of NaH2PO4-H3PO4 (10.0 mM, pH=5.0), respectively.Then, 10.0 mL of pNPG (2.0 mg/mL) was added to initiate the enzymatic reaction. After incubation for 60 s, the absorbance of product pNA was measured at 405 nm.Finally, the calibration curve was obtained. Subsequently, 30.0 mL of crude almond extract solution was diluted by 50.0 mL of NaH2PO4-H3PO4 (10.0 mM,pH ¼ 5.0). Then, 10.0 mL of pNPG (2.0 mg/mL) was added to initiate the enzymatic reaction. The absorbance of product pNA was measured at 405 nm after incubation for 60 s. The solution without pNPG was used as background solution. In this experiment, all assays were performed in triplicate.

来源文献：Chen GY, Zhang H, Yang FQ. A simple and portable method for β-Glucosidase activity assay and its inhibitor screening based on a personal glucose meter. Anal Chim Acta. 2021 Jan 15;1142:19-27. doi: 10.1016/j.aca.2020.10.047. Epub 2020 Oct 27. PMID: 33280697.

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