产品分类

ABTS Diammonium Salt
品牌:北京金优科技有限公司
货号 规格 包装/纯度 价格 货期 操作
JY-2025GR9110mM*1mL in Water 10mM*1mL in Water ≥98%
¥380.00
生物活性: 背景说明:ABTS Diammonium Salt是一种辣根过氧化物酶 (HRP) 的底物。
生物活性:ABTS diammonium salt 是一种辣根过氧化物酶 (HRP) 的底物。[1-2]
In Vitro:研究了使用ABTS作为HRP缀合物底物的酶联免疫吸附测定(ELISA)的微技术。在4个底物的比较研究中,即; 5-氨基水杨酸(5AS),邻苯二胺(OPD),O-联甲苯胺(OT)和ABTS,对于HRP的敏感性,ABTS是最敏感,最稳定,最具视觉效果的蓝绿色[ 1]。 ABTS是典型的过氧化物酶底物。为了纯化和表征,过氧化物酶阳性转化体在培养上清液中产生活性蛋白的条件下大规模培养(XL)。培养160小时后,获得相对于底物ABTS的55,000U / L的活性,并收获含有过氧化物酶的上清液。以ABTS为底物,当H2O2浓度超过0.125mM时,过氧化物酶活性显着下降,表明该酶被H2O2抑制。最大反应速率取决于测试的底物,其值在31.2和125μM之间[2]。
激酶实验:使用温度控制的多模板读数器或者在UV / Vis分光光度计中光度测定酶活性。通过添加酶引发反应。在25℃,在适当的基质波长下,在10分钟内测量酶活性。一个单位(1U)定义为每分钟转化1μmol底物的酶量。各种H2O2浓度(0-1250μM,酶浓度(0.27-54 nM)和底物浓度)用于测定酶活性。在pH3.8的100mM乙酸钠缓冲液和最终H2O2浓度下测定rPsaDyP对ABTS的活性。在420nm(ε42036,000Lmol-1 cm-1)下研究ABTS阳离子自由基的产生[2]。
数据来源文献:[1]. Matsuda H, et al. Evaluation of ELISA with ABTS, 2-2'-azino-di- (3-ethylbenzthiazoline sulfonic acid) , as the substrate of peroxidase and its application to the diagnosis of schistosomiasis. Jpn J Exp Med. 1984 Jun; 54 (3) :131-8.
[2]. Lauber C, et al. Identification, heterologous expression and characterization of a dye-decolorizing peroxidase of Pleurotus sapidus. AMB Express. 2017 Aug 23; 7 (1) :164
其它标识: EC:EINECS 250-396-6
MDL:MFCD00010404
SMILES:CCN1C(C=CC(S(=O)([O-])=O)=C2)=C2S/C1=N/N=C(SC3=C4C=CC(S(=O)([O-])=O)=C3)\N4CC.[NH4+].[NH4+]
InChIKey:OHDRQQURAXLVGJ-AXMZSLBLSA-N
InChI:InChI=1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;
PubChem CID:16240279
基本信息: CAS:30931-67-0
中文名称:2,2′-联氨-双(3-乙基苯并噻唑啉-6-磺酸)二胺盐
英文名称:ABTS Diammonium Salt
别名:AzBTS-(NH42);2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
分子式:C18H24N6O6S4
分子量:548.68
规格:10mM*1mL in DMSO ; 10mM*1mL in Water ; 500mg ; 1g
溶解性:Soluble in Water ≥50mg/mL;Soluble in DMSO ≥6mg/mL
纯度:≥98%
外观(性状):Light green to green Solid
储存条件:Powder:2-8℃,2 years;Insolvent(母液):-20℃,6 months;-80℃,1 year

产品详情

是一种辣根过氧化物酶 (HRP) 的底物。ABTS 经氧化后生成稳 定的蓝绿色阳离子 ABTS 自由基,在 405 nm 或 734 nm 处有最大吸收峰。被测物质加入 ABTS 自由基溶液后,所 含抗氧化成分能与 ABTS 自由基发生反应而使反应体系褪色,405 nm 的吸光度下降,在一定范围内其吸光度的变 化与自由基被清除的程度成正比。通过测定吸光度下降的程度来反映样本清除 ABTS 自由基的能力。ABTS 法可用于亲水性和亲脂性物质抗氧化能力测定。


使用本产品的应用案例(仅供参考

ABTS Free Radical Scavenging Assay.(ABTS 自由基清除测定)

案例1

To be specific, ABTS (25 mg) was added to the potassium persulfate (6.5 mL, 2.45 mmol/L) solution. Subsequently, the prepared ABTS solution was stored (14 h) at a constant temperature (25 ℃).The maintenance of the absorbance (734 nm, 0.70 ± 0.10) was confirmed before the experiments. Then, fresh MPN-GAs (1.0 mL, 1 mg/mL) and the prepared ABTS (3 mL) solution were subjected to an incubation process (60 min). Final ABTS scavenging activities were evaluated through the transformation of measured absorbance (734 nm). 

来源文献:Chen J, Chen X, Zhou G, Xu X. New insights into the ultrasound impact on covalent reactions of myofibrillar protein. Ultrason Sonochem. 2022 Mar;84:105973. doi: 10.1016/j.ultsonch.2022.105973. Epub 2022 Mar 3. PMID: 35272240; PMCID: PMC8913343.


案例2

ABTS was dissolved in 25 mM Tris, pH 7.0, to a 7 mM concentration. The ABTS radical cation (ABTS+·) was produced by mixing the ABTS stock solution with 2.45 mM potassium persulfate at a ratio of 1:1 (v:v) following 12−16 h of incubation in the dark (4 °C). The solution was then diluted with Tris buffer to an absorbance of 0.70 (±0.02) at 734 nm to form the test reagent. The test sample (3 μL) was then mixed with 237 μL of the diluted ABTS solution. The mixture reacted against light at room temperature for 30 min, and the absorbance was recorded at 734 nm on a ultraviolet spectrometer (AE). The blank was made from 3 μL of Tris buffer and 237 μL of diluted ABTS solution (AB). The percentage of inhibition of ABTS+· was calculated using the formula:

rate of inhibition=[ (AB-AE)/AB ] X 100

where AB is the absorbance of the blank sample and AE is the absorbance of the mixtures.

来源文献:Ma J, Yao Q, Chen X, Lv C, Zang J, Zhao G. Weak Binding of Epigallocatechin to α-Lactalbumin Greatly Improves Its Stability and Uptake by Caco-2 Cells. J Agric Food Chem. 2021 Aug 4;69(30):8482-8491. doi: 10.1021/acs.jafc.1c03427. Epub 2021 Jul 21. PMID: 34286590.




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