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背景说明：β-Amyloid (10-35)， amide 是一种含有 26 个氨基酸的多肽，由 β-Amyloid 肽的 10-35 残基组成。β-Amyloid (10-35) 是阿尔兹海默症淀粉样斑块 (Alzheimer’s disease) 的主要组成成分。
In Vitro：β-Amyloid (10-35) is selected based on the following considerations: (1) β-Amyloid (10-35) incorporates the core region， point mutations of which significantly obstruct fibril formation and have been used to generate inhibitors of fibrillogenesis; (2) β-Amyloid (10-35) retains the ability to add to bona fide Alzheimer’s plaques， in contrast to other truncated peptides， and forms fibrils morphologically similar to those of the full length peptide; (3) Of most importance， the full length peptide， Aβ(1-42)， is intractable for the controlled formation of fibrils from aqueous media because at the earliest time points， some of the peptide exists as an amorphous precipitate. In contrast， the use of β-Amyloid (10-35) allows the reproducible and controlled formation of fibrils from aqueous solutions， under defined conditions of pH， ionic strength， and peptide concentration and thus yields the required homogeneous fibrils.
参考文献：[1]. Benzinger TL， et al. Propagating structure of Alzheimer’s beta-amyloid(10-35) is parallel beta-sheet with residues in exact register. Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13407-12.
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