产品详情
基本操作（仅供参考）
1.接种干细胞：取对数生长期的细胞，按照2×104cells/cm2的细胞密度接种至包被后的培养器皿中，于37℃，5% CO2培养环境下培养至融合度90-100%，弃掉上清，加入成脂诱导分化培养基诱导液（含有地塞米松、胰岛素、IBMX和吲哚美辛/罗格列酮）。
2.细胞分化诱导：于37℃，5%CO2培养环境下培养约2-3天，更换为成脂诱导分化培养基维持液（只含有胰岛素）培养1天。
3.按照以上换液频率继续诱导（一般是3个循环，约14天左右），并注意观察细胞形态变化。根据细胞诱导形成的脂滴数量和大小，决定终止细胞诱导的时间，并进行染色鉴定。
4.细胞固定：吸去培养基,使用适量1×PBS清洗一次，弃去后取适量4%中性甲醛溶液覆盖培养器皿底面，室温固定30-60min，弃去固定液再使用1×PBS清洗两次。
5.油红O染色：加入适量工作液，静置染色30min；吸走油红O染色液，用1×PBS清洗两次，并加入适量1×PBS避免细胞干燥。
6.诱导评估：显微镜下观察成脂染色效果，并进行图像采集和诱导评估；诱导成功时，脂滴会与油红O染料结合后呈现红色或橘红色。
使用本产品的案例（仅供参考）
In Vitro
Cell（PDLSCs and GMSCs；1μM dexamethasone，0.2 mM indomethacin，0.01 g/l insulin，and 0.5 mM isobutyl-methylxanthine；4 weeks）
Multilineage differentiation assays were applied to detect the pluripotency of PDLSCs and GMSCs.For osteogenic or adipogenic induction, 2 × 105cells were seeded in 6-well dishes and cultured in osteogenic medium or adipogenic medium for 4 weeks,then the cells were fixed with 4% paraformaldehyde and stained with Alizarin Red (Solarbio, Beijing, China) or Oil Red O (Solarbio). ... ... The osteogenic medium was the complete culture medium supplemented with 10 nM dexamethasone(Solarbio), 10 mM β-glycerophosphate (Solarbio), and 50 mg/l ascorbic acid (Solarbio).The adipogenic medium was the complete culture medium supplemented with 1 μM dexamethasone (Solarbio), 0.2 mM indomethacin (Solarbio), 0.01 g/l insulin (Solarbio), and 0.5 mM isobutyl-methylxanthine(Solarbio).
来源文献：Jia L, Zhang Y, Ji Y, Li X, Xing Y, Wen Y, Huang H, Xu X. Comparative analysis of lncRNA and mRNA expression profiles between periodontal ligament stem cells and gingival mesenchymal stem cells. Gene. 2019 May 30;699:155-164. doi: 10.1016/j.gene.2019.03.015. Epub 2019 Mar 12. PMID: 30876821.
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