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背景说明：Antioxidant peptide A 是一种短肽。作用于癌细胞时，Antioxidant peptide A 的侧链有助于增强自由基清除活性。 Antioxidant peptide A is a short peptide, which contains alternative aromatic or sulfur-containing amino acid. The side chains of Antioxidant peptide A are believed to contribute to strong radical scavenging activities of peptides in the cancer cell.
In Vitro：The effects of 10-100 μM of Antioxidant peptide A (Pep-A) concentrations are studied on the superoxide dismutase (SOD) enzyme activity. The enzyme activity decreases by 0.5 and 0.7-folds at 10 and 50 μM Antioxidant peptide A concentrations, respectively, and increases by 1.79-folds at 100 μM Antioxidant peptide A treatment, indicating that this concentration can be ideal for the treatment on Y79 a, RB cells. Furthermore, the Antioxidant peptide A can be involved in decreasing the ROS by increasing the antioxidant enzyme activity. A similar increase in the antioxidative enzyme levels in the presence of Hoki skin antioxidative peptide in hepatocarcinoma cells is attributed to the peptide’s role in maintaining the redox balance in the cellular environment. Cell viability analysis results show that the Antioxidant peptide A shows no toxicity to cancerous (Y79) cells and non-cancerous cells even after 48 h of treatment. The Y79 RB cell viability ranges between 115 and 157 % and 111-126 % after 24 and 48 h of exposures with Antioxidant peptide A, respectively. The cancer cell death from the treatment of 10-100 μM GNPs concentration is studied.
参考文献：[1] Nilsson, AM. et al. Chem. Res. Toxicol. 18, 308 (2005);
[2] Ahlfors, SR. et al. Skin Pharmacol. Appl. Skin Physiol. 16, 59 (2003).
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