本套装内含1000×PMA(81μM)、Ionomycin(1.34mM)、Brefeldin A(10.6mM）的DMSO溶液和Monensin(2mM)的EtOH溶液各一支，每支溶液100μL。
在细胞内，PKC的激活可以引起下游众多蛋白激酶的磷酸化，形成级联反应，导致多种蛋白的表达，进而引起细胞的活化。PKC可以在DAG和Ca2+共同作用下被激活。PMA是一种有效的PKC激活剂；Ionomycin是一种有效的，选择性的钙离子载体。PMA常与Ionomycin联用，刺激免疫细胞活化，用于诱导体外细胞培养物产生细胞因子。
同时，也可加入蛋白转运抑制剂Monensin或Brefeldin A，抑制细胞因子外分泌，以准确分析细胞因子的表达水平。如果对分泌蛋白进行免疫检测（例如 ELISA、蛋白免疫印迹或多重蛋白检测），则无需使用Monensin或Brefeldin A对细胞进行处理。
实验案例（仅供参考） In Cell:
Flow Cytometric Analysis of the Percentages of Th17 Cells.（Spleen Mononuclear Cell/SMC, Thyroid Mononuclear Cell/TMC） Phorbol 12-myristate 13-acetate (PMA, Solarbio) and Ca-ionomycin (Solarbio) were added into the 12-well plates containing the SMC or TMC suspension, and brefeldin A (BFA, Solarbio) was added after 1 h followed by incubation for 3 h (total stimulation of 4 h). Cells were then collected, washed, and surface stained with APC-labeled CD4 antibody at 4℃ in the dark for 30 min. IC fixation buffer was then added followed by incubation at 4℃ in the dark for 20 min. Finally, cells were washed, resuspended in permeabilization buffer, and stained with PE-labeled IL-17A antibody at 4℃ in the dark for 30 min. Flow cytometric analyses were performed using a FACScanto flow cytometer. 来源文献：Liu H, Li Y, Zhu Y, Ma L, Xue H. Notch Signaling Pathway Promotes Th17 Cell Differentiation and Participates in Thyroid Autoimmune Injury in Experimental Autoimmune Thyroiditis Mice. Mediators Inflamm. 2023 Jan 6;2023:1195149. doi: 10.1155/2023/1195149. PMID: 36643586;PMCID: PMC9839414. | 
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