靶点：Others 通路：Others 背景说明：是一种具有生物活性的皂苷，可用于心血管，免疫，消化和神经系统等方向的研究。能够抑制ERK1/2和JNK的激活。 生物活性：Astragaloside IV， an active component isolated from Astragalus membranaceus， suppresses the activation of ERK1/2 and JNK， and downregulates matrix metalloproteases (MMP)-2， (MMP)-9 in MDA-MB-231 breast cancer cells.[1-4] In Vitro：Astragaloside IV (10， 20， 40 ng/mL) inhibits NSCLC cell growth， whereas low concentrations of astragaloside IV (1， 2.5， 5 ng/mL) has no obvious cytotoxicity on cell viability. Moreover， combined treatment with astragaloside IV significantly increases chemosensitivity to cisplatin in NSCLC cells. On the molecular level， astragaloside IV co-treatment significantly inhibits the mRNA and protein levels of B7-H3 in the presence of cisplatin[2]. Astragaloside IV inhibits the viability and invasive potential of MDA-MB-231 breast cancer cells， suppresses the activation of the mitogen activated protein kinase (MAPK) family members ERK1/2 and JNK， and downregulates matrix metalloproteases (MMP)-2 and -9[4]. In Vivo：Astragaloside IV (10， 20 mg/kg， p.o.) exhibits a potent ability to prevent cognitive deficits induced by transient cerebral ischemia and reperfusion. Astragaloside IV (10 mg/kg) and Astragaloside IV (20 mg/kg) can significantly decrease the levels of these cytokines compared to the Model group. Astragaloside IV significantly inhibits the level of TLR4 and its downstream proteins， suggesting that both MyD88-dependent and -independent pathways play important roles in the anti-inflammatory effects of Astragaloside IV. Astragaloside IV attenuates NLRP3 and cleaved-caspase-1 expression， and reduces Iba1 protein expression[1]. In the mice model， the high-dose astragaloside IV group has a significant increase in the 48-hour survival rate [60% (9/15) vs 13.3% (2/15)， P apoptosis of hepatocytes (P SOD[3]. 细胞实验：Cell viability is determined by CCK-8 assay. To be brief， cultured NSCLC cells are seeded into 96-well plates at the density of 4×104 (cells/well). Then 10 μL?well CCK8 solution is added and incubated in dark at 37°C for another 2 h. The absorbance is determined with the wavelength of 490 nm.[2] 动物实验：Transient cerebral ischemia and reperfusion is prepared by BCCAO， as BCCAO is considered an ideal model to study transient cerebral ischemia and reperfusion injury-mediated inflammatory response. Mice are randomLy divided into the Sham， Model， Astragaloside IV (10 mg/kg) and Astragaloside IV (20 mg/kg) treatment groups. The Astragaloside IV treatment groups are intragastrically administered 7 days before the surgery and terminated on the day of sacrifice. On the day of the surgery， Astragaloside IV is administrated 2 h prior to ischemia. The Sham-operated and Model groups are treated with distilled water. After the mice are anesthetized with an intraperitoneal injection of chloral hydrate (350 mg/kg)， the bilateral common carotid arteries are exposed and carefully separated with a small ventral neck incision and occluded twice (20 min each) with ligated surgical silk as described previously with minor modifications. There is a 10 min reperfusion period between the two occlusion periods (ischemia 20 min ? reperfusion 10 min ? ischemia 20 min). Sham-operated mice are subjected to the same surgical operation without the surgical silk ligation. Mouse body temperature is maintained at 37±0.5°C during the surgery with heating equipment until recovery from the anesthesia.[1] 激酶实验：Briefly， MDA-MB-231 cells treated as indicated or tumor tissues are harvested and lysed in Mg2+ lysis buffer containing 50 mM Tris (pH 7.5)， 10 mM MgCl2， 0.5 M NaCl， and protease inhibitor cocktail. Equal amounts of lysates are incubated with PAK-PBD beads at 4°C for 1 h. PAK-PBD beads are pelleted by centrifugation and washed with ish buffer containing 25 mM Tris (pH 7.5)， 30 mM MgCl2， 40 mM NaCl. Active Rac1 is detected by western blotting.[4] 数据来源文献：[1]. Li M， et al. Astragaloside IV attenuates cognitive impairments induced by transient cerebral ischemia and reperfusion in mice via anti-inflammatory mechanisms. Neurosci Lett. 2016 Dec 20. pii: S0304-3940(16)30994- [2]. He CS， et al. Astragaloside IV Enhances Cisplatin Chemosensitivity in Non-Small Cell Lung Cancer Cells Through Inhibition of B7-H3. Cell Physiol Biochem. 2016;40(5):1221-1229. Epub 2016 Dec 14. [3]. Liu L， et al. [Protective effect of astragaloside IV against acute liver failure in experimental mice]. Zhonghua Gan Zang Bing Za Zhi. 2016 Oct 20;24(10):772-777 [4]. Jiang K， et al. Astragaloside IV inhibits breast cancer cell invasion by suppressing Vav3 mediated Rac1/MAPK signaling. Int Immunopharmacol. 2016 Dec 5;42:195-20
基本信息：
CAS：84687-43-4 中文名称：黄芪甲苷(10mM in DMSO,无菌） 英文名称：Astragaloside Ⅳ(10mM in DMSO,Sterile） 分子式：C41H68O14 分子量：784.98 规格：1ml 溶解性：请根据自己的实验要求使用。 外观(性状)：无菌溶液 储存条件：Stroe at -20℃，6 months.
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