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矢车菊素-3-O-芸香糖苷
品牌：北京金优科技有限公司
货号 规格 包装/纯度 价格 货期 操作
JY-2025BOL461mg 1mg HPLC≥98%
¥940.00
收藏产品 大包装询价
其它标识： EC：EINECS 242-528-6
MDL：MFCD00135404
SMILES：CC1C(C(C(C(O1)OCC2C(C(C(C(O2)OC3=CC4=C(C=C(C=C4[O+]=C3C5=CC(=C(C=C5)O)O)O)O)O)O)O)O)O)O.[Cl-]
InChIKey：ADZHXBNWNZIHIX-XYGAWYNKSA-N
InChI：InChI=1S/C27H30O15.ClH/c1-9-19(32)21(34)23(36)26(39-9)38-8-18-20(33)22(35)24(37)27(42-18)41-17-7-12-14(30)5-11(28)6-16(12)40-25(17)10-2-3-13(29)15(31)4-10;/h2-7,9,18-24,26-27,32-37H,8H2,1H3,(H3-,28,29,30,31);1H/t9-,18+,19-,20+,21+,22-,23+,24+,26+,27+;/m0./s1
PubChem CID：29231
基本信息： CAS：18719-76-1
中文名称：矢车菊素-3-O-芸香糖苷
英文名称：Cyanidin-3-O-Rutinoside
别名：凯拉花青;Cyanidin 3-O-rutinoside chloride
纯度：HPLC≥98%
分子式：C27H31O15Cl
分子量：630.99
外观(性状)：结晶或粉末
规格：1mg
溶解性：Slightly Soluble in Methanol
储存条件：Store at -20℃，2 years.
溶解性： Slightly Soluble in Methanol

产品详情

使用本产品的案例（仅供参考）


HPLC

。。。the supernatant was filtered with a microporous membrane (0.45mm) for highperformance liquid chromatography (HPLC) analysis, and Comatex C18 column (250 mm x 4.6 mm, 5 mm), column temperature 30 ℃. The elution system consisted of a mobile phase A (ultra-pure water) with a gradient elution procedure at a flow rate of 1.0 mL·min-1, injection volume of 10.0 mL, column temperature of 30 ℃, and detection wavelength of 520 nm.

来源文献：Yao L, Liang D, Xia H, Pang Y, Xiao Q, Huang Y, Zhang W, Pu C, Wang J, Lv X. Biostimulants promote the accumulation of carbohydrates and biosynthesis of anthocyanins in 'Yinhongli'plum. Front Plant Sci. 2023 Jan 6;13:1074965. doi: 10.3389/fpls.2022.1074965. PMID: 36684717;PMCID: PMC9854126.


UPLC-MS/MS

The analytical conditions were as follows, UPLC: column, C18 (1.8 μm, 2.1 mm × 100 mm);the mobile phase was consisted of solvent A, pure water with 0.1% formic acid, and solvent B, acetonitrile. Sample measurements were performed with a gradient program that employed the starting conditions of 95% A, 5% B. Within 9 min, a linear gradient to 5% A, 95% B was programmed, and a composition of 5% A, 95% B was kept for 1min. Subsequently, a composition of 95% A, 5.0% B was adjusted within 1.10 min and kept for 2.9 min. The column oven was set to 40 ℃. The injection volume was 4 μL.

The ESI source operation parameters were as follows: ion source, turbo spray;source temperature 550 ℃;ion spray voltage (IS) 5500 V (positive ion mode)/-4500 V (negative ion mode);ion source gas I (GSI), gas II(GSII), curtain gas (CUR) were set at 50, 60, and 30.0 psi, respectively;the collision gas (CAD) was high. Instrument tuning and mass calibration were performed with 10 and 100 μM polypropylene glycol solutions in QQQ and LIT modes, respectively. QQQ scans were acquired as MRM experiments with collision gas (nitrogen) set to 5 psi. DP and CE for individual MRM transitions were done with further DP and CE optimization. A specific set of MRM transitions were monitored for each period according to the metabolites eluted within this period.

文献来源：[1] Xiao Y , Li K , Zhang H ,et al.The profile of buckwheat tannins based on widely targeted metabolome analysis and pharmacokinetic study of ellagitannin metabolite urolithin A[J].LWT, 2022, 156:113069-.DOI:10.1016/j.lwt.2022.113069.


HPLC

The phenolic acid contents were also measured usingHigh Performance Liquid Chromatography.Chromatographic separation was conductedwith an C18 column (4.6 mm × 250 mm,5 mm) and a binary solvent system of (A) methanol (0.1%H3PO4) and (B) water (0.1% H3PO4). The elution wasperformed at a flow rate of 1.0 mL/min and the columntemperature was maintained at 30℃. The contents of phenolicacids [CGA, neochlorogenic acid (NCGA), cryptochlorogenicacid (CCGA), p-coumaryl quinic acid, and caffeoyl shikimicacid] were detected at 320 nm and the contents of anthocyanin(cyanidin-3-O-glucoside chloride and cyanidin 3-O-rutinosidechloride) were quantified at 525 nm.

来源文献：Su Z, Jia H, Sun M, Cai Z, Shen Z, Zhao B, Li J, Ma R, Yu M, Yan J. Integrative analysis of the metabolome and transcriptome reveals the molecular mechanism of chlorogenic acid synthesis in peach fruit. Front Nutr. 2022 Jul 19;9:961626. doi: 10.3389/fnut.2022.961626. PMID: 35928835;PMCID: PMC9344011.


UPLC-QTOT-MS

The C18 (2.1 × 100 mm, 1.7 µm) was kept at 30 ℃. The injection volume was 1 µL, and the elution was completed in 18 min with a flow rate of 0.3 mL/min. Solvents A (water + 0.1% formic acid) and B (acetonitrile) were used in the following gradients: 0–2 min (5–10% B), 2–10 min (10–20% B), 10–15 min (20–40% B), 15–17 min (40–70% B), and 17–18 min (70–100% B). The PDA spectra for phenolic compounds were measured at 320 nm and 350 nm. For MS analysis, anthocyanins were analyzed in the positive ion (PI) mode;other phenolic compounds were analyzed in the negative ion (NI) modes. The MS parameters were as follows: source temperature of 120 ℃, desolvation temperature of 250 ℃(400℃ in the positive ion), cone gas flow 50 L/h, desolvation gas flow 600 L/h (800 L/h in the positive ion), source capillary of 3.0 kV. The MS analysis was performed using mass scanning from m/z 50 to 1,500.

来源文献：[1] Xiang Z , Lin C , Zhu Y ,et al.Phytochemical profiling of antioxidative polyphenols and anthocyanins in the wild plant Campanumoea lancifolia (Roxb.) Merr[J].International Journal of Food Properties, 2021, 24(1):105-114.DOI:10.1080/10942912.2020.1867570.


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