| Elisa信息： |
Swiss Prot：Q9NZT1
Gene ID：51806
检测原理：Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人CALML5/CLSP单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的人CALML5/CLSP会与酶标板上的包被抗体充分结合；洗板后加入HRP酶标记的抗人CALML5/CLSP抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的人CALML5/CLSP发生特异性结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的人CALML5/CLSP，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的人CALML5/CLSP浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中人CALML5/CLSP的浓度。
背景说明：Calmodulin-like protein 5 is a protein that in humans is encoded by the CALML5 gene.
This gene encodes a novel calcium binding protein expressed in the epidermis and related to the calmodulin family of calcium binding proteins. Functional studies with recombinant protein demonstrate it does bind calcium and undergoes a conformational change when it does so. Abundant expression is detected only in reconstructed epidermis and is restricted to differentiating keratinocytes. In addition, it can associate with transglutaminase 3, shown to be a key enzyme in the terminal differentiation of keratinocytes.
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